Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors
Identifieur interne : 000009 ( France/Analysis ); précédent : 000008; suivant : 000010Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors
Auteurs : Young-Jun Park [États-Unis] ; Alexandra C. Walls [États-Unis] ; Zhaoqian Wang [États-Unis] ; Maximillian M. Sauer [États-Unis] ; Wentao Li [Pays-Bas] ; M. Alejandra Tortorici [États-Unis, France] ; Berend-Jan Bosch [Pays-Bas] ; Frank Dimaio [États-Unis] ; David Veesler [États-Unis]Source :
- Nature Structural & Molecular Biology [ 1545-9993 ] ; 2019.
Descripteurs français
- KwdFr :
- Acides sialiques (), Acides sialiques (métabolisme), Cartographie d'interactions entre protéines, Conformation des glucides, Conformation des protéines, Coronavirus du syndrome respiratoire du Moyen-Orient (), Cryomicroscopie électronique, Dipeptidyl peptidase 4 (), Dipeptidyl peptidase 4 (métabolisme), Dipeptidyl peptidase 4 (ultrastructure), Domaines protéiques, Glycoprotéine de spicule des coronavirus (), Glycoprotéine de spicule des coronavirus (métabolisme), Glycoprotéine de spicule des coronavirus (ultrastructure), Humains, Hémagglutination virale, Liaison aux protéines, Modèles moléculaires, Relation structure-activité, Sites de fixation.
- MESH :
- métabolisme : Acides sialiques, Dipeptidyl peptidase 4, Glycoprotéine de spicule des coronavirus.
- ultrastructure : Dipeptidyl peptidase 4, Glycoprotéine de spicule des coronavirus.
- Acides sialiques, Cartographie d'interactions entre protéines, Conformation des glucides, Conformation des protéines, Coronavirus du syndrome respiratoire du Moyen-Orient, Cryomicroscopie électronique, Dipeptidyl peptidase 4, Domaines protéiques, Glycoprotéine de spicule des coronavirus, Humains, Hémagglutination virale, Liaison aux protéines, Modèles moléculaires, Relation structure-activité, Sites de fixation.
English descriptors
- KwdEn :
- Binding Sites, Carbohydrate Conformation, Cryoelectron Microscopy, Dipeptidyl Peptidase 4 (chemistry), Dipeptidyl Peptidase 4 (metabolism), Dipeptidyl Peptidase 4 (ultrastructure), Hemagglutination, Viral, Humans, Middle East Respiratory Syndrome Coronavirus (chemistry), Models, Molecular, Protein Binding, Protein Conformation, Protein Domains, Protein Interaction Mapping, Sialic Acids (chemistry), Sialic Acids (metabolism), Spike Glycoprotein, Coronavirus (chemistry), Spike Glycoprotein, Coronavirus (metabolism), Spike Glycoprotein, Coronavirus (ultrastructure), Structure-Activity Relationship.
- MESH :
- chemical , chemistry : Dipeptidyl Peptidase 4, Sialic Acids, Spike Glycoprotein, Coronavirus.
- chemical , metabolism : Dipeptidyl Peptidase 4, Sialic Acids, Spike Glycoprotein, Coronavirus.
- chemical , ultrastructure : Dipeptidyl Peptidase 4, Spike Glycoprotein, Coronavirus.
- chemistry : Middle East Respiratory Syndrome Coronavirus.
- Binding Sites, Carbohydrate Conformation, Cryoelectron Microscopy, Hemagglutination, Viral, Humans, Models, Molecular, Protein Binding, Protein Conformation, Protein Domains, Protein Interaction Mapping, Structure-Activity Relationship.
Abstract
The Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe and often lethal respiratory illness in humans, and no vaccines or specific treatments are available. Infections are initiated via binding of the MERS-CoV spike (S) glycoprotein to sialosides and dipeptidyl-peptidase 4 (the attachment and entry receptors, respectively). To understand MERS-CoV engagement of sialylated receptors, we determined the cryo-EM structures of S in complex with 5-
Url:
DOI: 10.1038/s41594-019-0334-7
PubMed: 31792450
PubMed Central: 7097669
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000416
- to stream Pmc, to step Curation: 000416
- to stream Pmc, to step Checkpoint: 000169
- to stream PubMed, to step Corpus: 000347
- to stream PubMed, to step Curation: 000347
- to stream PubMed, to step Checkpoint: 000366
- to stream Ncbi, to step Merge: 002417
- to stream Ncbi, to step Curation: 002417
- to stream Ncbi, to step Checkpoint: 002417
- to stream Main, to step Merge: 000368
- to stream Main, to step Curation: 000365
- to stream Main, to step Exploration: 000365
- to stream France, to step Extraction: 000009
Links to Exploration step
PMC:7097669Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors</title><author><name sortKey="Park, Young Jun" sort="Park, Young Jun" uniqKey="Park Y" first="Young-Jun" last="Park">Young-Jun Park</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Walls, Alexandra C" sort="Walls, Alexandra C" uniqKey="Walls A" first="Alexandra C." last="Walls">Alexandra C. Walls</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Wang, Zhaoqian" sort="Wang, Zhaoqian" uniqKey="Wang Z" first="Zhaoqian" last="Wang">Zhaoqian Wang</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Sauer, Maximillian M" sort="Sauer, Maximillian M" uniqKey="Sauer M" first="Maximillian M." last="Sauer">Maximillian M. Sauer</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Li, Wentao" sort="Li, Wentao" uniqKey="Li W" first="Wentao" last="Li">Wentao Li</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000000120346234</institution-id><institution-id institution-id-type="GRID">grid.5477.1</institution-id><institution>Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine,</institution><institution>Utrecht University,</institution></institution-wrap>Utrecht, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Utrecht</wicri:regionArea></affiliation></author><author><name sortKey="Tortorici, M Alejandra" sort="Tortorici, M Alejandra" uniqKey="Tortorici M" first="M. Alejandra" last="Tortorici">M. Alejandra Tortorici</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2353 6535</institution-id><institution-id institution-id-type="GRID">grid.428999.7</institution-id><institution>Institut Pasteur, Unité de Virologie Structurale,</institution></institution-wrap>Paris, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Paris</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2353 6535</institution-id><institution-id institution-id-type="GRID">grid.428999.7</institution-id><institution>CNRS UMR 3569, Unité de Virologie Structurale,</institution></institution-wrap>Paris, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Paris</wicri:regionArea></affiliation></author><author><name sortKey="Bosch, Berend Jan" sort="Bosch, Berend Jan" uniqKey="Bosch B" first="Berend-Jan" last="Bosch">Berend-Jan Bosch</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000000120346234</institution-id><institution-id institution-id-type="GRID">grid.5477.1</institution-id><institution>Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine,</institution><institution>Utrecht University,</institution></institution-wrap>Utrecht, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Utrecht</wicri:regionArea></affiliation></author><author><name sortKey="Dimaio, Frank" sort="Dimaio, Frank" uniqKey="Dimaio F" first="Frank" last="Dimaio">Frank Dimaio</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Veesler, David" sort="Veesler, David" uniqKey="Veesler D" first="David" last="Veesler">David Veesler</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31792450</idno><idno type="pmc">7097669</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7097669</idno><idno type="RBID">PMC:7097669</idno><idno type="doi">10.1038/s41594-019-0334-7</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000416</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000416</idno><idno type="wicri:Area/Pmc/Curation">000416</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000416</idno><idno type="wicri:Area/Pmc/Checkpoint">000169</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000169</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:31792450</idno><idno type="wicri:Area/PubMed/Corpus">000347</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000347</idno><idno type="wicri:Area/PubMed/Curation">000347</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000347</idno><idno type="wicri:Area/PubMed/Checkpoint">000366</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000366</idno><idno type="wicri:Area/Ncbi/Merge">002417</idno><idno type="wicri:Area/Ncbi/Curation">002417</idno><idno type="wicri:Area/Ncbi/Checkpoint">002417</idno><idno type="wicri:doubleKey">1545-9993:2019:Park Y:structures:of:mers</idno><idno type="wicri:Area/Main/Merge">000368</idno><idno type="wicri:Area/Main/Curation">000365</idno><idno type="wicri:Area/Main/Exploration">000365</idno><idno type="wicri:Area/France/Extraction">000009</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors</title><author><name sortKey="Park, Young Jun" sort="Park, Young Jun" uniqKey="Park Y" first="Young-Jun" last="Park">Young-Jun Park</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Walls, Alexandra C" sort="Walls, Alexandra C" uniqKey="Walls A" first="Alexandra C." last="Walls">Alexandra C. Walls</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Wang, Zhaoqian" sort="Wang, Zhaoqian" uniqKey="Wang Z" first="Zhaoqian" last="Wang">Zhaoqian Wang</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Sauer, Maximillian M" sort="Sauer, Maximillian M" uniqKey="Sauer M" first="Maximillian M." last="Sauer">Maximillian M. Sauer</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Li, Wentao" sort="Li, Wentao" uniqKey="Li W" first="Wentao" last="Li">Wentao Li</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000000120346234</institution-id><institution-id institution-id-type="GRID">grid.5477.1</institution-id><institution>Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine,</institution><institution>Utrecht University,</institution></institution-wrap>Utrecht, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Utrecht</wicri:regionArea></affiliation></author><author><name sortKey="Tortorici, M Alejandra" sort="Tortorici, M Alejandra" uniqKey="Tortorici M" first="M. Alejandra" last="Tortorici">M. Alejandra Tortorici</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2353 6535</institution-id><institution-id institution-id-type="GRID">grid.428999.7</institution-id><institution>Institut Pasteur, Unité de Virologie Structurale,</institution></institution-wrap>Paris, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Paris</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2353 6535</institution-id><institution-id institution-id-type="GRID">grid.428999.7</institution-id><institution>CNRS UMR 3569, Unité de Virologie Structurale,</institution></institution-wrap>Paris, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Paris</wicri:regionArea></affiliation></author><author><name sortKey="Bosch, Berend Jan" sort="Bosch, Berend Jan" uniqKey="Bosch B" first="Berend-Jan" last="Bosch">Berend-Jan Bosch</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000000120346234</institution-id><institution-id institution-id-type="GRID">grid.5477.1</institution-id><institution>Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine,</institution><institution>Utrecht University,</institution></institution-wrap>Utrecht, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Utrecht</wicri:regionArea></affiliation></author><author><name sortKey="Dimaio, Frank" sort="Dimaio, Frank" uniqKey="Dimaio F" first="Frank" last="Dimaio">Frank Dimaio</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Veesler, David" sort="Veesler, David" uniqKey="Veesler D" first="David" last="Veesler">David Veesler</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000122986657</institution-id><institution-id institution-id-type="GRID">grid.34477.33</institution-id><institution>Department of Biochemistry,</institution><institution>University of Washington,</institution></institution-wrap>Seattle, WA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Seattle</wicri:cityArea></affiliation></author></analytic><series><title level="j">Nature Structural & Molecular Biology</title><idno type="ISSN">1545-9993</idno><idno type="eISSN">1545-9985</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Binding Sites</term><term>Carbohydrate Conformation</term><term>Cryoelectron Microscopy</term><term>Dipeptidyl Peptidase 4 (chemistry)</term><term>Dipeptidyl Peptidase 4 (metabolism)</term><term>Dipeptidyl Peptidase 4 (ultrastructure)</term><term>Hemagglutination, Viral</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus (chemistry)</term><term>Models, Molecular</term><term>Protein Binding</term><term>Protein Conformation</term><term>Protein Domains</term><term>Protein Interaction Mapping</term><term>Sialic Acids (chemistry)</term><term>Sialic Acids (metabolism)</term><term>Spike Glycoprotein, Coronavirus (chemistry)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Spike Glycoprotein, Coronavirus (ultrastructure)</term><term>Structure-Activity Relationship</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides sialiques ()</term><term>Acides sialiques (métabolisme)</term><term>Cartographie d'interactions entre protéines</term><term>Conformation des glucides</term><term>Conformation des protéines</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term><term>Cryomicroscopie électronique</term><term>Dipeptidyl peptidase 4 ()</term><term>Dipeptidyl peptidase 4 (métabolisme)</term><term>Dipeptidyl peptidase 4 (ultrastructure)</term><term>Domaines protéiques</term><term>Glycoprotéine de spicule des coronavirus ()</term><term>Glycoprotéine de spicule des coronavirus (métabolisme)</term><term>Glycoprotéine de spicule des coronavirus (ultrastructure)</term><term>Humains</term><term>Hémagglutination virale</term><term>Liaison aux protéines</term><term>Modèles moléculaires</term><term>Relation structure-activité</term><term>Sites de fixation</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Dipeptidyl Peptidase 4</term><term>Sialic Acids</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Dipeptidyl Peptidase 4</term><term>Sialic Acids</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Dipeptidyl Peptidase 4</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides sialiques</term><term>Dipeptidyl peptidase 4</term><term>Glycoprotéine de spicule des coronavirus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Dipeptidyl peptidase 4</term><term>Glycoprotéine de spicule des coronavirus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Binding Sites</term><term>Carbohydrate Conformation</term><term>Cryoelectron Microscopy</term><term>Hemagglutination, Viral</term><term>Humans</term><term>Models, Molecular</term><term>Protein Binding</term><term>Protein Conformation</term><term>Protein Domains</term><term>Protein Interaction Mapping</term><term>Structure-Activity Relationship</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Acides sialiques</term><term>Cartographie d'interactions entre protéines</term><term>Conformation des glucides</term><term>Conformation des protéines</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Cryomicroscopie électronique</term><term>Dipeptidyl peptidase 4</term><term>Domaines protéiques</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Hémagglutination virale</term><term>Liaison aux protéines</term><term>Modèles moléculaires</term><term>Relation structure-activité</term><term>Sites de fixation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">The Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe and often lethal respiratory illness in humans, and no vaccines or specific treatments are available. Infections are initiated via binding of the MERS-CoV spike (S) glycoprotein to sialosides and dipeptidyl-peptidase 4 (the attachment and entry receptors, respectively). To understand MERS-CoV engagement of sialylated receptors, we determined the cryo-EM structures of S in complex with 5-<italic>N</italic>-acetyl neuraminic acid, 5-<italic>N</italic>-glycolyl neuraminic acid, sialyl-Lewis<sup>X</sup>, α2,3-sialyl-<italic>N</italic>-acetyl-lactosamine and α2,6-sialyl-<italic>N</italic>-acetyl-lactosamine at 2.7–3.0 Å resolution. We show that recognition occurs via a conserved groove that is essential for MERS-CoV S-mediated attachment to sialosides and entry into human airway epithelial cells. Our data illuminate MERS-CoV S sialoside specificity and suggest that selectivity for α2,3-linked over α2,6-linked receptors results from enhanced interactions with the former class of oligosaccharides. This study provides a structural framework explaining MERS-CoV attachment to sialoside receptors and identifies a site of potential vulnerability to inhibitors of viral entry.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sabir, Js" uniqKey="Sabir J">JS Sabir</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Menachery, Vd" uniqKey="Menachery V">VD Menachery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Menachery, Vd" uniqKey="Menachery V">VD Menachery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hu, B" uniqKey="Hu B">B Hu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gui, M" uniqKey="Gui M">M Gui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kirchdoerfer, Rn" uniqKey="Kirchdoerfer R">RN Kirchdoerfer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shang, J" uniqKey="Shang J">J Shang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shang, J" uniqKey="Shang J">J Shang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, Ac" uniqKey="Walls A">AC Walls</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, Ac" uniqKey="Walls A">AC Walls</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiong, X" uniqKey="Xiong X">X Xiong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, Y" uniqKey="Yuan Y">Y Yuan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, Ac" uniqKey="Walls A">AC Walls</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kirchdoerfer, Rn" uniqKey="Kirchdoerfer R">RN Kirchdoerfer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, W" uniqKey="Song W">W Song</name></author><author><name sortKey="Gui, M" uniqKey="Gui M">M Gui</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Xiang, Y" uniqKey="Xiang Y">Y Xiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tortorici, Ma" uniqKey="Tortorici M">MA Tortorici</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, Ac" uniqKey="Walls A">AC Walls</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pallesen, J" uniqKey="Pallesen J">J Pallesen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Raj, Vs" uniqKey="Raj V">VS Raj</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Park, Je" uniqKey="Park J">JE Park</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Millet, Jk" uniqKey="Millet J">JK Millet</name></author><author><name sortKey="Whittaker, Gr" uniqKey="Whittaker G">GR Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Earnest, Jt" uniqKey="Earnest J">JT Earnest</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Earnest, Jt" uniqKey="Earnest J">JT Earnest</name></author><author><name sortKey="Hantak, Mp" uniqKey="Hantak M">MP Hantak</name></author><author><name sortKey="Park, Je" uniqKey="Park J">JE Park</name></author><author><name sortKey="Gallagher, T" uniqKey="Gallagher T">T Gallagher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Y" uniqKey="Yang Y">Y Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stencel Baerenwald, Je" uniqKey="Stencel Baerenwald J">JE Stencel-Baerenwald</name></author><author><name sortKey="Reiss, K" uniqKey="Reiss K">K Reiss</name></author><author><name sortKey="Reiter, Dm" uniqKey="Reiter D">DM Reiter</name></author><author><name sortKey="Stehle, T" uniqKey="Stehle T">T Stehle</name></author><author><name sortKey="Dermody, Ts" uniqKey="Dermody T">TS Dermody</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiong, X" uniqKey="Xiong X">X Xiong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiong, X" uniqKey="Xiong X">X Xiong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, G" uniqKey="Lu G">G Lu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Widagdo, W" uniqKey="Widagdo W">W Widagdo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vergara Alert, J" uniqKey="Vergara Alert J">J Vergara-Alert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barlan, A" uniqKey="Barlan A">A Barlan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyer, B" uniqKey="Meyer B">B Meyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hulswit, Rjg" uniqKey="Hulswit R">RJG Hulswit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Campbell, Mg" uniqKey="Campbell M">MG Campbell</name></author><author><name sortKey="Veesler, D" uniqKey="Veesler D">D Veesler</name></author><author><name sortKey="Cheng, A" uniqKey="Cheng A">A Cheng</name></author><author><name sortKey="Potter, Cs" uniqKey="Potter C">CS Potter</name></author><author><name sortKey="Carragher, B" uniqKey="Carragher B">B Carragher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, X" uniqKey="Yu X">X Yu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suzuki, Y" uniqKey="Suzuki Y">Y Suzuki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Millet, Jk" uniqKey="Millet J">JK Millet</name></author><author><name sortKey="Whittaker, Gr" uniqKey="Whittaker G">GR Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiong, X" uniqKey="Xiong X">X Xiong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wickramasinghe, In" uniqKey="Wickramasinghe I">IN Wickramasinghe</name></author><author><name sortKey="De Vries, Rp" uniqKey="De Vries R">RP de Vries</name></author><author><name sortKey="Grone, A" uniqKey="Grone A">A Grone</name></author><author><name sortKey="De Haan, Ca" uniqKey="De Haan C">CA de Haan</name></author><author><name sortKey="Verheije, Mh" uniqKey="Verheije M">MH Verheije</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bakkers, Mj" uniqKey="Bakkers M">MJ Bakkers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Cm" uniqKey="Chan C">CM Chan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chu, H" uniqKey="Chu H">H Chu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Widjaja, I" uniqKey="Widjaja I">I Widjaja</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suloway, C" uniqKey="Suloway C">C Suloway</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tegunov, D" uniqKey="Tegunov D">D Tegunov</name></author><author><name sortKey="Cramer, P" uniqKey="Cramer P">P Cramer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zivanov, J" uniqKey="Zivanov J">J Zivanov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Punjani, A" uniqKey="Punjani A">A Punjani</name></author><author><name sortKey="Rubinstein, Jl" uniqKey="Rubinstein J">JL Rubinstein</name></author><author><name sortKey="Fleet, Dj" uniqKey="Fleet D">DJ Fleet</name></author><author><name sortKey="Brubaker, Ma" uniqKey="Brubaker M">MA Brubaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zivanov, J" uniqKey="Zivanov J">J Zivanov</name></author><author><name sortKey="Nakane, T" uniqKey="Nakane T">T Nakane</name></author><author><name sortKey="Scheres, Shw" uniqKey="Scheres S">SHW Scheres</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scheres, Sh" uniqKey="Scheres S">SH Scheres</name></author><author><name sortKey="Chen, S" uniqKey="Chen S">S Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goddard, Td" uniqKey="Goddard T">TD Goddard</name></author><author><name sortKey="Huang, Cc" uniqKey="Huang C">CC Huang</name></author><author><name sortKey="Ferrin, Te" uniqKey="Ferrin T">TE Ferrin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brown, A" uniqKey="Brown A">A Brown</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Emsley, P" uniqKey="Emsley P">P Emsley</name></author><author><name sortKey="Lohkamp, B" uniqKey="Lohkamp B">B Lohkamp</name></author><author><name sortKey="Scott, Wg" uniqKey="Scott W">WG Scott</name></author><author><name sortKey="Cowtan, K" uniqKey="Cowtan K">K Cowtan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dimaio, F" uniqKey="Dimaio F">F DiMaio</name></author><author><name sortKey="Leaver Fay, A" uniqKey="Leaver Fay A">A Leaver-Fay</name></author><author><name sortKey="Bradley, P" uniqKey="Bradley P">P Bradley</name></author><author><name sortKey="Baker, D" uniqKey="Baker D">D Baker</name></author><author><name sortKey="Andre, I" uniqKey="Andre I">I Andre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dimaio, F" uniqKey="Dimaio F">F DiMaio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frenz, B" uniqKey="Frenz B">B Frenz</name></author><author><name sortKey="Walls, Ac" uniqKey="Walls A">AC Walls</name></author><author><name sortKey="Egelman, Eh" uniqKey="Egelman E">EH Egelman</name></author><author><name sortKey="Veesler, D" uniqKey="Veesler D">D Veesler</name></author><author><name sortKey="Dimaio, F" uniqKey="Dimaio F">F DiMaio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frenz, Bdr" uniqKey="Frenz B">BDR Frenz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, A" uniqKey="Walls A">A Walls</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Ry" uniqKey="Wang R">RY Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Vb" uniqKey="Chen V">VB Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barad, Ba" uniqKey="Barad B">BA Barad</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Agirre, J" uniqKey="Agirre J">J Agirre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Krissinel, E" uniqKey="Krissinel E">E Krissinel</name></author><author><name sortKey="Henrick, K" uniqKey="Henrick K">K Henrick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goddard, Td" uniqKey="Goddard T">TD Goddard</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>France</li><li>Pays-Bas</li><li>États-Unis</li></country><region><li>Washington (État)</li></region></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Park, Young Jun" sort="Park, Young Jun" uniqKey="Park Y" first="Young-Jun" last="Park">Young-Jun Park</name></region><name sortKey="Dimaio, Frank" sort="Dimaio, Frank" uniqKey="Dimaio F" first="Frank" last="Dimaio">Frank Dimaio</name><name sortKey="Sauer, Maximillian M" sort="Sauer, Maximillian M" uniqKey="Sauer M" first="Maximillian M." last="Sauer">Maximillian M. Sauer</name><name sortKey="Tortorici, M Alejandra" sort="Tortorici, M Alejandra" uniqKey="Tortorici M" first="M. Alejandra" last="Tortorici">M. Alejandra Tortorici</name><name sortKey="Veesler, David" sort="Veesler, David" uniqKey="Veesler D" first="David" last="Veesler">David Veesler</name><name sortKey="Walls, Alexandra C" sort="Walls, Alexandra C" uniqKey="Walls A" first="Alexandra C." last="Walls">Alexandra C. Walls</name><name sortKey="Wang, Zhaoqian" sort="Wang, Zhaoqian" uniqKey="Wang Z" first="Zhaoqian" last="Wang">Zhaoqian Wang</name></country><country name="Pays-Bas"><noRegion><name sortKey="Li, Wentao" sort="Li, Wentao" uniqKey="Li W" first="Wentao" last="Li">Wentao Li</name></noRegion><name sortKey="Bosch, Berend Jan" sort="Bosch, Berend Jan" uniqKey="Bosch B" first="Berend-Jan" last="Bosch">Berend-Jan Bosch</name></country><country name="France"><noRegion><name sortKey="Tortorici, M Alejandra" sort="Tortorici, M Alejandra" uniqKey="Tortorici M" first="M. Alejandra" last="Tortorici">M. Alejandra Tortorici</name></noRegion><name sortKey="Tortorici, M Alejandra" sort="Tortorici, M Alejandra" uniqKey="Tortorici M" first="M. Alejandra" last="Tortorici">M. Alejandra Tortorici</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000009 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000009 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= France
|étape= Analysis
|type= RBID
|clé= PMC:7097669
|texte= Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/France/Analysis/RBID.i -Sk "pubmed:31792450" \
| HfdSelect -Kh $EXPLOR_AREA/Data/France/Analysis/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |